EI2302 ANALYTICAL INSTRUMENTS Syllabus - EIE 5th Semester - Anna University


EI2302                                             ANALYTICAL INSTRUMENTS                                         LT P C
3 0 0 3
AIM
The course is designed to equip the students with an adequate knowledge of a number of analytical tools which are useful for clinical analysis in hospitals, drugs and pharmaceutical laboratories and above all for environmental Pollution Monitoring.

OBJECTIVES
i.          To provide various techniques and methods of analysis which occur in the various regions of the spectrum. These are the powerful tools used in Clinical and Research laboratories.
ii.          To give unique methods of separation of closely similar materials, the most powerful being gas chromatography.
iii.         To study important methods of  analysis of  industrial gases. Awareness and control of pollution in the environment is of vital importance.
iv.        To bring out the latest ideas on ion-selective electrodes as well as biosensors which have potential applications in medical field, food and beverage industries.
v.        To provide the important electromagnetic resonance and microscopic methods of analysis.
Further they are both sensitive and specific and often are characterized by good accuracy. NMR & ESR and microscopic techniques are useful in structure determination.

UNIT I            COLORIMETRY AND SPECTROPHOTOMETRY                                                     9
Special methods of analysis Beer-Lambert law – Colorimeters UV-Visible spectrophotometers Single and double beam instruments Sources and detectors – IR Spectrophotometers Types Attenuated total reflectance flame photometers – Atomic absorption spectrophotometers Sources and detectors – FTIR spectrophotometers Flame emission photometers Fluorescence spectrophotometer

UNIT II           CHROMATOGRAPHY                                                                                                9
Different techniques – Gas chromatography – Detectors Liquid chromatographs – Applications High-pressure liquid chromatographs Applications.


UNIT III           INDUSTRIAL GAS ANALYZERS AND POLLUTION MONITORING
INSTRUMENTS                                                                                                            9
Types  of  gas  analyzers   Oxygen,  NO and  H2S  types,  IR  analyzers,  thermal  conductivity analyzers, analysis based on ionization of gases. Air pollution due to carbon monoxide, hydrocarbons, nitrogen oxides, sulphur dioxide estimation - Dust and smoke measurements.

UNIT IV           pH METERS AND DISSOLVED COMPONENT ANALYZERS                                  9
Principle  of  pH  measurement,  glass  electrodes,  hydrogen  electrodes,  reference  electrodes, selective ion electrodes, ammonia electrodes, cyclic voltametry, biosensors, dissolved oxygen analyzer Sodium analyzer – Silicon analyzer.


UNIT V           ELECTRO MAGNETIC RESONANCE AND MICROSCOPIC TECHNIQUES            9
NMR Basic principles NMR spectrometer - Applications. Electron spin Resonance spectroscopy
Basic principles, Instrumentation and applications. Scanning Electron Microscope (SEM), - Basic principles, Instrumentation and applications. Transmission Electron Microscope (TEM) Basic principles Instrumentation and applications.  Mass spectrometers Different types Applications.

TOTAL : 45 PERIODS

TEXT BOOKS:
1.  G.W. Ewing, Instrumental Methods of Analysis, McGraw Hill, 1992.
2.  R.K.Jain, Mechanical and Industrial Measurements, Khanna Publishers, New Delhi, 1999
3.  H.H. Willard, L.L.  Merritt,  J.A. Dean, F.A. Settle,  Instrumental  Methods  of Analysis,  CBS
publishing & distribution, 1995.

REFERENCES:
1.  Robert D. Braun, Introduction to Instrumental Analysis, McGraw Hill, Singapore, 1987.
2.  R.S. Khandpur, ‘Handbook of Analytical Instruments, Tata McGraw Hill publishing Co. Ltd.,
2003.
3.  Liptak, B.G, Process Measurement and Analysis, Chilton Book Company, 1995










By Vinoth
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